Genome assembly and annotation of D. hansenii strain
Debaryomyces hansenii is a halotolerant yeast species known for its resilience in extreme environments, including high salinity and oxidative stress [1]. These characteristics make it a promising candidate for space biology research, where microbial robustness is essential for long-duration missions [2]. Recent studies by the European Space Agency (ESA) have highlighted the importance of fungi in space [3]. Importantly, its ability to produce vitamin B2 and vitamin D makes it particularly suitable to study as a supplement for space diets. [4,5] However, the strain of D. hansenii remains genomically uncharacterized. To enable functional studies of this strain, this project will generate a de novo genome assembly which will be further use to construct a custom protein database for mass spectrometry (MS)-based proteomics.
Objective
Annotate and assemble the genome of D. hansenii strain from raw sequencing reads, further predict protein-coding genes.
Approach
- perform quality control and genome assembly with the paired-end read
- perform assembled quality evaluation and genome annotation to predicted gene models and protein sequences
- (optional) use predicted proteome for MS run identification.
References
- Comparison of molecular and metabolomic methods as characterization tools of Debaryomyces hansenii cheese isolates. https://doi.org/10.1016/j.fm.2009.03.009
- Perchlorate-specific proteomic stress responses of Debaryomyces hansenii could enable microbial survival in Martian brines. https://doi.org/10.1111/1462-2920.16152
- ESA - Turning up gravity for space fungi study
- The potential role of extremophiles as food supplements in extraterrestrial bioscience engineering – ScienceOpen
- Microbial supplements for extraterrestrial environments : Exploration of microbial food supplements to support human life on Mars – ScienceOpen
- MGI Tech. (2024). DNBSEQ™ Sequencing Technology Overview. https://en.mgitech.cn/Home/Applications/index/id/20.html